12. Immunological methods for testing innate and adaptive humoral factors – tests for complement and other serum proteins (agglutination, precipitation, complement-fixation, and labeled immune reactions) – principles, clinical application, and interpretation.
Topic 12 of 14 in the MU Plovdiv syllabus for Clinical Immunology (Medicine, Year 4). The free AceMed note covers it in 1 section, about 73 words (2 min of reading), with 13 flashcards.
In this note
IMMUNOLOGICAL METHODS OF EXAMINATION:
IMMUNOLOGICAL METHODS OF EXAMINATION:
Notes:
ANA (Antinuclear Antibody) = autoantibodies detected by Indirect Immunofluorescence (IFA).
Rheumatoid Factor (RF) can be detected by ELISA, latex agglutination, or IFA.
IgE (allergy antibody) usually measured by ELISA.
HIV antibodies: screening by ELISA, confirmation by Western blot.
PSA (Prostate Specific Antigen) measured by ELISA or related immunoassays. Also is elevated in smokers and can be evaluated in prostatitis and prostate cancer.
Western blot and Immunoblot are synonyms and used for HIV
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