Cytology · MU Sofia

Slide 19. Fibrous-compact regular (collagenous) connective tissue tendon, H&E

Explains how to recognise a tendon under H&E staining as dense regular connective tissue, with thick parallel collagen bundles stained pink and rows of flattened tenocytes with long dark nuclei between them. Covers the sparse cell and vessel content, the star-shaped fibroblasts seen in cross-section, and a comparison with smooth muscle, whose nuclei sit inside pink cytoplasm rather than between fibres.

Inside: 1 section · 1 min

Topic 108 of 127 in the MU Sofia syllabus for Cytology (Dental Medicine, Year 1). The free AceMed note covers it in 1 section, about 102 words (1 min of reading).

In this note

  1. Introduction

Introduction

A tendon is dense regular connective tissue: thick collagen bundles all running in one direction, so it resists pull along that line.

Stain

H&E: collagen pink, nuclei blue-purple.

How to recognise it

  • Thick, parallel, slightly wavy pink collagen bundles.
  • Rows of flattened fibroblasts (tenocytes) between the bundles, with long, dark nuclei in lines.
  • Very few cells and vessels.
  • In cross-section, the fibroblasts look star-shaped between the round bundles.

Don't confuse it with: smooth muscle, whose nuclei lie inside the cells, in the middle of pink cytoplasm, and are not squeezed between fibres.

Theory: topics 42 (intercellular fibres) and 45 (fibrous connective tissue).

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