Slide 24. Peripheral blood smear from a human, Romanowski-Giemsa staining
Explains how to recognise a peripheral blood smear stained with Romanowsky–Giemsa, describing the staining colours of nuclei and erythrocytes. It goes on to identify erythrocytes and platelets, then sets out a table comparing the five leukocyte types by their share of white cells, nucleus shape and cytoplasm or granule appearance.
Inside: 1 section · 1 table · 1 min
Topic 113 of 127 in the MU Sofia syllabus for Cytology (Dental Medicine, Year 1). The free AceMed note covers it in 1 section, about 75 words (1 min of reading).
In this note
Introduction
Introduction
A thin layer of blood spread on a slide shows every type of blood cell.
Stain
Romanowsky–Giemsa: nuclei purple, erythrocytes pink-orange, cytoplasm and granules coloured by cell type.
How to recognise it
Most cells are erythrocytes: round, pink-orange discs about 7.5 µm across, with no nucleus and a paler centre.
Platelets: small purple fragments, often in clumps.
Leukocytes are told apart by their nucleus and granules (table below).
Theory: topics 51–56 (blood and blood cells).
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