Slide 38. Organogenesis of chicken - Toluidine blue
Walks through a toluidine blue section of an early chick embryo, showing how to recognise the neural tube, notochord, somites, intermediate and lateral plate mesoderm with the coelom, dorsal aortae, gut endoderm and surface ectoderm. Notes the stain colouring and points to the related gastrulation and germ layer theory.
Inside: 1 section · 1 min
Topic 127 of 127 in the MU Sofia syllabus for Cytology (Dental Medicine, Year 1). The free AceMed note covers it in 1 section, about 126 words (1 min of reading).
In this note
Introduction
Introduction
A cross-section of an early chick embryo shows the germ layers beginning to form organs. Exactly what you see depends on the stage and the level of the section.
Stain
Toluidine blue: cells blue, tissues of different density in different shades.
How to recognise it
The neural tube in the midline at the top: thick, dark neuroepithelium around a narrow canal.
The notochord just below it: a small round rod of pale, vacuolated cells.
Somites on both sides of the neural tube: blocks of paraxial mesoderm.
Further out, intermediate mesoderm and lateral plate mesoderm split into two layers with the coelom between them.
Paired dorsal aortae, the endoderm of the gut, and surface ectoderm covering the embryo.
Theory: topics 82–85 (gastrulation, germ layers and their derivatives).
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