Slide 10. Spherical nuclei of the spinal ganglion, H&E
Explains how to recognise a spinal ganglion slide stained with H&E, where neurons show large spherical pale nuclei with loose chromatin and a prominent central nucleolus. Covers the staining colours, the appearance of neuron groups, the ring of satellite-cell nuclei around each neuron, and how to avoid confusing them with the flattened dark nuclei of satellite and Schwann cells.
Inside: 1 section · 1 min
Topic 99 of 127 in the MU Sofia syllabus for Cytology (Dental Medicine, Year 1). The free AceMed note covers it in 1 section, about 88 words (1 min of reading).
In this note
Introduction
Introduction
Neurons of the spinal (dorsal root) ganglion show a typical spherical nucleus.
Stain
H&E: nuclei blue-purple, cytoplasm pink.
How to recognise it
Large round neuron cell bodies in groups.
A big, round, pale nucleus in the centre of each, with loose (euchromatic) chromatin.
A dark, prominent nucleolus in the middle of the nucleus (an "owl's eye" look).
A ring of small satellite-cell nuclei around each neuron.
Don't confuse it with: the small, dark, flattened nuclei of the satellite and Schwann cells around the neurons.
Theory: topic 22 (nucleus).
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