Slide 9. Lipids subcutaneous adipose tissue, Sudan III
Explains why fat is shown on a frozen section rather than paraffin, and how Sudan III stains lipid droplets orange-red. Describes the large round or polygonal adipocytes with a single lipid droplet, the thin cytoplasmic rim and the peripherally pushed nucleus, plus the septa and capillaries between cells. Notes that the same tissue looks empty in H&E.
Inside: 1 section · 1 min
Topic 98 of 127 in the MU Sofia syllabus for Cytology (Dental Medicine, Year 1). The free AceMed note covers it in 1 section, about 97 words (1 min of reading).
In this note
Introduction
Introduction
The slide demonstrates lipids, which are dissolved in routine paraffin sections. It is cut frozen so the fat stays in the cells.
Stain
Sudan III dissolves into the fat and colours it orange-red.
How to recognise it
Large round or polygonal cells, each filled by one big orange-red lipid droplet.
A thin rim of cytoplasm, with a flattened nucleus pushed to the edge (signet-ring look).
Thin connective tissue septa and capillaries between the cells.
Don't confuse it with: the same tissue in H&E, where the fat cells look empty.
Theory: topics 3 (histochemistry) and 46 (adipose tissue).
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