Hygiene and Epidemiology · MU Sofia

8. Proteins, fats and carbohydrates. Nutritional assessment, requirements, functions, sources.

Covers the roles of proteins, fats and carbohydrates in human nutrition, taking each macronutrient in turn: functions, essential amino acids, sources, recommended intakes and health effects of deficiency and excess. Protein quality is explained with high- and low-quality examples and the BV and PDCAAS measures, and carbohydrates are split into simple and complex types. It closes with dietary fibre and a brief note on energy expenditure, including doubly labelled water as the reference method.

Inside: 6 sections · 5 labelled pictures · 28 flashcards · 4 min

Topic 8 of 21 in the MU Sofia syllabus for Hygiene and Epidemiology (Dental Medicine, Year 3). The free AceMed note covers it in 6 sections, about 442 words (4 min of reading), with 28 flashcards.

In this note

  1. Role and importance of proteins, fats and carbohydrates in human nutrition. Dietary fibers.
  2. Fats (Lipids)
  3. Carbohydrates
  4. Dietary Fibers
  5. Energy expenditure and caloric value of food.
  6. Energy Expenditure

Role and importance of proteins, fats and carbohydrates in human nutrition. Dietary fibers.

1. Proteins

Functions:

  • Structural: Build and repair tissues (muscles, skin, hair, nails, organs)
  • Enzymatic: Act as enzymes for biochemical reactions
  • Hormonal: Some proteins function as hormones (insulin, glucagon)
  • Transport: Hemoglobin transports oxygen; other proteins transport nutrients
  • Immune: Antibodies fight infections
  • Energy: 4 kcal/g if carbs and fats are insufficient

Essential Amino Acids:

  • Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine

Sources:

  • Animal: Meat, fish, eggs, milk, dairy
  • Plant: Legumes, nuts, seeds, soy products

Recommended Intake:

  • Adults: 0.8–1.0 g/kg body weight/day (higher in athletes, pregnancy, or illness)

Health Significance:

  • Adequate intake: Supports growth, tissue repair, immune function
  • Deficiency: Muscle wasting, edema, growth retardation, immune dysfunction
  • Excess: Kidney strain, potential calcium loss

Protein Quality: How well a protein provides all essential amino acids (EAAs) and is digestible.

High-Quality (Complete):

  • All EAAs present, well absorbed
  • Examples: Eggs, milk, meat, fish, soy, quinoa

Low-Quality (Incomplete):

  • Lacking one or more EAAs
  • Examples: Wheat, rice, corn, most legumes

BV (Biological Value) and PDCAAS are tools used to measure protein quality:

  • BV measures how efficiently the body retains absorbed protein for growth and maintenance.
  • PDCAAS evaluates protein quality by combining amino acid composition and digestibility.

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